Antigen analysis of cultivable Reiter and Nichols treponemes.
نویسندگان
چکیده
منابع مشابه
Antigenic relationships of 14 treponemes demonstrated by immunofluorescence.
Immunofluorescence was used as an aid in the antigenic grouping of 14 cultivable treponemes. Antisera were prepared versus each treponemal strain, and the antiglobulins were conjugated with fluorescein isothiocyanate. A common antigen-antibody system, detected in the strains studied, was removed by absorption of each conjugate with Reiter or Borrelia vincentii treponemes. Thus, five categories ...
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Bactericidal studies of antibiotics on treponemes have been limited to a small group of organisms and to a limited number of antimicrobial agents. Eagle and Musselman (1944) determined the sensitivity in vitro of the non-virulent Reiter treponeme to penicillin G. Penicillin concentrations of between 01 and 0-25 units/ml. medium killed 90 to 99 per cent. of the spirochaetes in 8 to 12 hrs. Penic...
متن کاملImmune responses to soluble antigens of treponemes.
Sonicates from five cultivable treponemes were used as antigens in delayed hypersensitivity tests and macrophage inhibition assays. Immunodiffusion analysis showed that the sonicate comprised two major antigenic components which were not distinguishable in the skin tests. The sonicate antigens elicited significant cell-mediated immunity in guinea-pigs. Treponema refringens biotype Nichols prove...
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Three genetically distinct groups of treponemes have been identified by saturation reassociation assays using 125I-labeled treponemal DNAs. The three groups are (i) virulent Treponema pallidum (Nichols strain), (ii) T. phagedenis and its biotypes Reiter and Kazan 5, and (iii) T. refringens biotypes Nichols and Noguchi. There is no detectable DNA sequence homology (less than 5%) among the three ...
متن کاملIsolation and antigenic characteristics of axial filaments from the Reiter Treponeme.
Axial filaments were isolated and purified from Reiter treponemes after detergent solubilization of the cells" outer envelope. The axial filaments were separated from the spirochetal cells by shearing, purified by density gradient centrifugation, and fragmented by ultrasonication. Acrylamide gel electrophoresis of dissociated filaments revealed two major protein bands. Gel diffusion precipitin ...
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عنوان ژورنال:
- The British journal of venereal diseases
دوره 48 6 شماره
صفحات -
تاریخ انتشار 1972